| Types | DnaRegion
|
| Roles | Plasmid
plasmid
|
| Sequences | BBa_J100310_sequence (Version 1)
|
Description
J100287 is a plasmid that could be used in the positive selection phase of Phage Assisted Continuous Evolution (PACE) to evolve riboswitches. Gene III, the gene necessary for phage survival, and GFP, a reporter gene, are separated by a stop codon at the end of gene III. J100310 had two bases, specifically "TA", in the stop codon between the genes removed, forming a translational fusion of the two proteins. While both gene III and GFP should be produced in J100287 since the GFP gene has its own ribosome binding site, J100310 is another option to try for PACE.
Notes
The exact sequence of the stop codon between gene III and GFP in J100287 is "TAA", with the second "A" also being the first base of the "ATG" start codon at the beginning of GFP. Therefore, two bases (the "TA" in the stop codon) had to be removed for the gene III and GFP sequences to be in the same reading frame.
Source
The sequence for this part comes from J100287, which itself comes from the J100265 (pJC173b) plasmid that the Campbell synthetic biology lab at Davidson College received from the Liu lab at Harvard University.
