BBa_J10065

BBa_J10065 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J10065

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Malcolm Campbell

Date created: 2012-01-10 12:00:00

Date modified: 2015-08-31 04:08:24

LuxI from V. fischeri C0061 with LVA tag removed

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J10065_sequence (Version 1)

Description

Synthesizes 3OC6HSL, which binds to LuxR.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

This part is based on C0061 but the LVA degradation tag has been removed.

Notes

removed LVA tag

Source

registry part, synthesized de novo

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	1,3	feature/start, start_codon
double stop	580,585	stop_codon, feature/stop
luxI V. fischeri	1,579	feature/protein, CDS

Other Properties

• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J10065/1

Member of these Collections

• iGEM Parts Registry