Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2012-01-10 12:00:00
Date modified: 2015-08-31 04:08:24
sacB minus RBS (based on K322921)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J10067_sequence (Version 1) |
Description
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.sacB encodes the Bacillus subtilis levansucrase, which catalyses hydrolysis of sucrose and synthesis of levans (high molecular weight fructose polymers). It is lethal to gram-negative bacteria E-coli.
It works with cat as an alternative method for inserting BioBricks into the genome by using homologous recombination rather than restriction digestion. SacB is used as a negative selection marker, which allows to insert genes onto the chromosomes without leaving a selection marker. The method can thus be reused indefinitely.
The protocol for BRIDGE can be found on the Edinburgh 2010 igem wiki.
http://2010.igem.org/Team:Edinburgh/Project/Protocol