BBa_J107013

BBa_J107013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J107013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lorenzo Pasotti

Date created: 2010-06-05 11:00:00

Date modified: 2015-08-31 04:08:24

HSL inducible RiPS generator and ORF expresser

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_J107013_sequence (Version 1)

Description

Overview'

If this part is contained in a vector that has a transcriptional terminator downstream of the BioBrick Suffix (e.g. pSB1A3 or pSB4C5 which have the E. coli his terminator BBa_B0053), an ORF can be quickly assembled and tested under the control of the lux HSL inducible promoter (BBa_R0062).


Usage

The ORF can simply be a coding sequence (from ATG to a stop codon), without any RBS or transcriptional terminator. BBa_J107013 can be used as follows:

*digest the plasmid containing BBa_J107013 with SpeI-PstI;
*digest the ORF with XbaI-PstI;
*ligate the two DNA fragments and transform them in E. coli;
*let the transformed cells grow and induce them with [[3OC6HSL|3OC₆HSL]] to trigger the expression of the ORF.


Compatibility
For the reasons explained above, this part must be used in vectors that have a transcriptional terminator downstream of the BioBrick Suffix (e.g. pSB1A3 or pSB4C5). Otherwise, the ORF must be ligated to a transcriptional terminator (e.g. BBa_B0015) before being assembled with BBa_J107013.

Notes

We used BioBrick Standard Assembly.

Source

BBa_F2620, sent by MIT;

BBa_B0034, 2009 DNA Distribution.

Components

Access	Instance	Definition
public	BBa_F2620	BBa_F2620
public	BBa_B0034	BBa_B0034

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_F2620	1,1061	BBa_F2620
BBa_B0034	1070,1081	BBa_B0034

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J107013/1

Member of these Collections

• iGEM Parts Registry