BBa_J119010

BBa_J119010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caleb J. Carr

Date created: 2011-08-02 11:00:00

Date modified: 2015-08-31 04:08:26

EcoRI-BsaI-START-BsmBI-PstI

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J119010_sequence (Version 1)

Description

This is a 64 bp sequence designed for the START portion of Golden Gate Assembly of Hamiltonian Path Problems.
1. Use BsaI to free up new START word (GTCC) on 5??? end that allows ligation to new HPP vector.
2. Use BsmBI to free up half-edge word (GTGG) on 3??? end that allows ligation to any Gene1 half-edge.
3. In between these is a J23100 promoter.
4. Flanked by EcoRI and PstI sticky ends for cloning.

Notes

This is the start sequence to be annealed to the HPP vector followed by genesof choice and followed by the finish sequence.

Source

Synthetic Oligos.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
EcoRI	1,6	sequence_feature, feature/dna
BsaI	7,12	sequence_feature, feature/dna
START	14,17	feature/misc, sequence_feature
J23100	18,52	sequence_feature, feature/dna
PstI	64,69	sequence_feature, feature/dna
BsmBI	58,63	feature/dna, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119010/1

Member of these Collections

• iGEM Parts Registry