BBa_J119024

BBa_J119024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vivek Mutalik
Date created: 2011-12-21 12:00:00
Date modified: 2015-10-01 11:42:24

BD18 bicistronic translational junction - Medium effiency



    • Details

    Types
    DnaRegion

    Roles
    Translational_Unit

    engineered_region

    Sequences BBa_J119024_sequence (Version 1)

    Description

    The BD18 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest.

    Notes

    The BD18 bicistronic translational junction was designed to give reproducible and consistent expression for various genes of interest.

    Source

    Synthetic DNA.

    Sequence Annotation Location Component / Role(s)
    leader RBS
    Start
    Stop
    Start
    GOI RBS
    		19,27
    33,35
    84,86
    86,88
    71,79
    		feature/rbs ribosome_entry_site
    feature/start start_codon
    stop_codon feature/stop
    feature/start start_codon
    ribosome_entry_site feature/rbs

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J119024/1
     

    Attachments

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