BBa_J119027

BBa_J119027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Vivek Mutalik

Date created: 2011-12-21 12:00:00

Date modified: 2015-10-01 11:43:50

BD21 bicistronic translational junction - High efficiency

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_J119027_sequence (Version 1)

Description

The BD21 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest.

Notes

The bicistronic translational junction was designed by Team Leader Vivek Mutalik at BioFAB http://www.biofab.org. It is is intended to give reproducible and consistent expression for various genes of interest.

Source

Synthetic DNA.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	33,35	feature/start, start_codon
Start	86,88	start_codon, feature/start
leader RBS	19,27	ribosome_entry_site, feature/rbs
GOI RBS	71,79	feature/rbs, ribosome_entry_site
Stop	84,86	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119027/1

Member of these Collections

• iGEM Parts Registry