BBa_J119039

BBa_J119039 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119039
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2012-01-02 12:00:00
Date modified: 2015-08-31 04:08:26

GFP2-FINISH



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_J119039_sequence (Version 1)

Description

Half-edge for use in assembly of Hamiltonian Path Problem graphs. Composed of GFP2 half-edge and FINISH half-edge. Contains GFP gene-specific word and FINISH word that become available for ligation upon digestion with BsaI during Golden Gate Assembly.

Notes

Contains GFP gene-specific word and FINISH word that become available for ligation upon digestion with BsaI during Golden Gate Assembly.

Source

Ligated from cloned parts.

Sequence Annotation Location Component / Role(s)
EcoRI
PstI
BsaI forward
BsaI reverse
GFP word
FINISH word
GFP2
1,6
466,471
7,12
460,465
14,17
455,458
18,382
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119039/1