BBa_J119042

BBa_J119042 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119042
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2012-01-04 12:00:00
Date modified: 2015-08-31 04:08:26

pTet-BD18-SacB



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_J119042_sequence (Version 1)

    Description

    Promoter pTet drives SacB expression via the BD18 bicistronic translational junction engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. SacB can be used for negative selection.

    Notes

    The bicistronic translational junction was designed by Team Leader Vivek Mutalik at BioFAB [1]. It is is intended to give reproducible and consistent expression for various genes of interest.

    Source

    De novo DNA synthesis.

    Sequence Annotation Location Component / Role(s)
    pTet
    BD18 bicistronic junction
    SacB
    		1,52
    53,142
    140,1564
    		feature/misc sequence_feature
    feature/rbs ribosome_entry_site
    feature/cds CDS

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J119042/1
     

    Attachments

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