BBa_J119055

BBa_J119055 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119055
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michel Conn
Date created: 2012-01-26 12:00:00
Date modified: 2015-08-31 04:08:26

pLacI-RBS-T7 RNAP (2SAT mutant 1)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_J119055_sequence (Version 1)

Description

T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (a??? or b) and (a or c???).

Notes

The frameshift mutation is of the form (a??? or b) and (a or c???).

Source

Existing part and synthetic oligos.

Sequence Annotation Location Component / Role(s)
pLacI
B0030
T7 RNAP
22 nt frameshift insertion
1,56
68,82
89,2810
115,126
promoter feature/promoter
feature/rbs ribosome_entry_site
CDS feature/cds
feature/mutation sequence_alteration
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119055/1