BBa_J119055

BBa_J119055 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119055

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michel Conn

Date created: 2012-01-26 12:00:00

Date modified: 2015-08-31 04:08:26

pLacI-RBS-T7 RNAP (2SAT mutant 1)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J119055_sequence (Version 1)

Description

T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (a??? or b) and (a or c???).

Notes

The frameshift mutation is of the form (a??? or b) and (a or c???).

Source

Existing part and synthetic oligos.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pLacI	1,56	promoter, feature/promoter
B0030	68,82	feature/rbs, ribosome_entry_site
T7 RNAP	89,2810	CDS, feature/cds
22 nt frameshift insertion	115,126	feature/mutation, sequence_alteration

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119055/1

Member of these Collections

• iGEM Parts Registry