BBa_J119057

BBa_J119057 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119057
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Barta
Date created: 2012-01-26 12:00:00
Date modified: 2015-08-31 04:08:26

pLacI-RBS-T7 RNAP (2SAT mutant 3)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_J119057_sequence (Version 1)

Description

T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (b or c)and(a??? or b???).

Notes

The frameshift mutation is of the form (b or c)and(a??? or b???).

Source

Existing part and synthetic oligos.

Sequence Annotation Location Component / Role(s)
pLacI
B0030
T7 RNAP
22 nt frameshift mutation
1,56
68,82
89,2810
115,126
feature/promoter promoter
ribosome_entry_site feature/rbs
CDS feature/cds
feature/mutation sequence_alteration
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119057/1