Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2012-01-27 12:00:00
Date modified: 2015-08-31 04:08:26
pSB1A2-BR
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_J119061_sequence (Version 1) |
Description
Plasmid pSB1A2 with BsaI site removed from Ampicillin resistance gene. For use in Golden Gate Assembly with BsaI.Notes
Original amino acid sequence of Amplicillin gene retained.Source
PCR mutagenesis of pSB1A2.| Sequence Annotation | Location | Component / Role(s) |
| Suffix Prefix VR VF2 rep (pMB1) (rev) Ampicillin resistance gene (rev) Terminator c -> a (BsaI site removed) | 1,21 2058,2079 79,98 1940,1959 183,797 957,1817 2029,2048 1101,1101 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site CDS feature/cds feature/cds CDS feature/stem_loop stem_loop point_mutation feature/s_mutation |