BBa_J119076

BBa_J119076 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J119076

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Carr

Date created: 2012-03-05 12:00:00

Date modified: 2015-08-31 04:08:27

GGA HPP Start Edge

Details

• Types: DnaRegion
• Roles: sequence_feature; Other

Description

This part is a component of an assembly method. It is flanked by two sets of type 2 restriction enzymes. EcoRI, PstI, BsaI, and BbsI. Inside of the restriction enzyme sequences it contains two 4 letter sticky words specific for our assembly process and between those a promoter (J23100) and RBS (BD18).

Notes

The sticky words were chosen specifically to contain at least 3 C or G nucleotides to promote optimal thermodynamic binding.

Source

de novo

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119076/1

Member of these Collections

• iGEM Parts Registry