Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Carr
Date created: 2012-03-09 12:00:00
Date modified: 2015-08-31 04:08:27
J23100-BD18 used for GGA/HPP2.0 (RFP Edge)
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_J119080_sequence (Version 1) |
Description
This part is one of several similar parts designed in our lab to serve as "Edges" in a Hamiltonian Path Problem experiment. This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a Promoter and RBS.Notes
This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a Promoter and RBS.Source
de novo| Sequence Annotation | Location | Component / Role(s) |
| EcoRI cuts left BsaI cuts right BsaI word (Node Exit RFP) J23100 BD18 BbsI word (Node Word) BbsI cuts left PstI cuts right | 1,6 7,12 14,17 18,52 53,140 137,140 142,147 148,153 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/dna feature/promoter promoter ribosome_entry_site feature/rbs sequence_feature feature/dna sequence_feature feature/misc sequence_feature feature/misc |