BBa_J119082

BBa_J119082 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119082
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Carr
Date created: 2012-03-09 12:00:00
Date modified: 2015-08-31 04:08:27

J23100-BD18 for GGA/HPP (Lac-Z)



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_J119082_sequence (Version 1)

Description

This part is one of several similar parts designed in our lab to serve as "Edges" in a Hamiltonian Path Problem experiment. This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a Promoter and RBS.

Notes

This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a Promoter and RBS.

Source

de novo

Sequence Annotation Location Component / Role(s)
EcoRI cuts left
BsaI cuts right
BsaI word (Node Exit-Lac-Z)
J23100
BD18
BbsI word (Node Word)
BbsI cuts left
PstI cuts right
1,6
7,12
14,17
18,52
53,140
137,140
142,147
148,153
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/dna
promoter feature/promoter
ribosome_entry_site feature/rbs
sequence_feature feature/dna
feature/misc sequence_feature
feature/misc sequence_feature
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119082/1