Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Lab Fall 2012 2:00
Date created: 2013-01-07 12:00:00
Date modified: 2015-08-31 04:08:27
pTac with mutations
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J119132_sequence (Version 1) |
Description
This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. This promoter sequence is without the -10 element and the -35 element is present. Golden Gate Assembly with annealed oligos with J100091 as the receiving plasmid and Bsa I sticky ends. This promoter inserted at the double terminator region. There was a loss of function with this promoter. The RFP was unable to be turned on.Notes
Oligonucleotides.Source
The oligonucleotides needed to have sticky ends compatible with those produced by BsaI digestion of J100091 so that Golden Gate Assembly could be used.| Sequence Annotation | Location | Component / Role(s) |
| -10 region | 27,32 | feature/dna sequence_feature |