Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Lab Fall 2012 2:00
Date created: 2013-01-07 12:00:00
Date modified: 2015-08-31 04:08:27
pTac with mutations
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J119134_sequence (Version 1) |
Description
This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. Mutation of pTac Promoter substitution of 2nd nt from an A to a T in the -35 regionNotes
Oligonucleotides.Source
The oligonucleotides needed to have sticky ends compatible with those produced by BsaI digestion of J100091 so that Golden Gate Assembly could be used.| Sequence Annotation | Location | Component / Role(s) |
| -35 region -10 region | 11,15 33,37 | sequence_feature feature/dna feature/dna sequence_feature |