Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sachith Dharmasena and Todd Eckdahl
Date created: 2013-02-08 12:00:00
Date modified: 2015-08-31 04:08:27
Theophylline Riboswitch - tetracycline resistance protein
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J119140_sequence (Version 1) |
Description
This device contains a synthetic riboswitch that can be used in E. coli. In the presence of theophylline, the transcription of the TetA gene occurs, but in the absence of theophylline, very little transcription takes place. This device contains a modified T5 promoter, which, in the absence of cymR, acts as a strong constitutive promoter. The ribosomal binding site is contained in the riboswitch.This riboswitch is modified from riboswitch D as described in the paper by Topp et al.
References: Shana Topp, Colleen M. K. Reynoso, Jessica C. Seeliger, Ian S. Goldlust, Shawn K. Desai, Doroth??e Murat, Aimee Shen, Aaron W. Puri, Arash Komeili, Carolyn R. Bertozzi, June R. Scott, and Justin P. Gallivan. Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species. Applied and Environmental Microbiology, 76:23, 7881-7884. December 2010.
Notes
BsaI sites were introduced into PCR products, but are not present in the final construct.Source
Constructed using BsaI GGA of PCR products from J100065 and J31007.| Sequence Annotation | Location | Component / Role(s) |
| T5 promoter lac operator riboswitch Start codon TetA tetracycline resistance gene Stop codon | 1,53 54,74 123,182 160,162 160,1350 1351,1353 | promoter feature/promoter operator feature/operator stem_loop feature/stem_loop feature/start start_codon feature/cds CDS stop_codon feature/stop |