Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brandon Grieshaber and Todd Eckdahl
Date created: 2013-07-28 11:00:00
Date modified: 2015-08-31 04:08:28
pSB1A2-BR-HS-T-RNA-I
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_J119312_sequence (Version 1) |
Description
This vector is pSB1A2 with its BsaI site removed (BR = BsaI Removed), so it can be used in BsaI Golden Gate Assembly. It also has been verified to have an T at position -1 of the RNA I gene in the pMB1 origin of replication. This site has been identified as a hotspot for mutation of the pMB1 origin (Lin-Chao et al. 1992).Notes
NoneSource
Junction Golden Gate Assembly was used to mutate position -1 of the RNA I gene in the pMB1 origin of replication.| Sequence Annotation | Location | Component / Role(s) |
| Suffix rep (pMB1) (rev) APr (rev) RNA I hotspot T txn terminator Prefix | 1,21 183,797 957,1817 639,639 2029,2048 2058,2079 | feature/BioBrick engineered_region CDS feature/cds CDS feature/cds feature/mutation sequence_alteration feature/stem_loop stem_loop feature/BioBrick engineered_region |