BBa_J119320

BBa_J119320 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119320
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2013 Tuesday 8:00 lab
Date created: 2013-11-18 12:00:00
Date modified: 2015-08-31 04:08:28

pTac mutant -33 A



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J119320_sequence (Version 1)

Description

This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2013. It was cloned into the pClone Green vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation the T at position -33 to A. The mutation had little effect on the function of the promoter (see Experience page).

Notes

Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.

Source

Synthetic oligonucleotides.

Sequence Annotation Location Component / Role(s)
-35 region
-10 region
T to A mutation
7,12
29,34
8,8
feature/promoter promoter
promoter feature/promoter
feature/mutation sequence_alteration
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119320/1