Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-01-13 12:00:00
Date modified: 2015-08-31 04:08:28
Ammeline Biosensor with Riboswitch M6C + RFP
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J119329_sequence (Version 1) |
Description
The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.Notes
None.Source
Golden Gate Assembly with BsaI of J119137 and PCR product of RFP.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter M6C'' Ammeline riboswitch RFP | 19,55 73,188 183,866 | feature/promoter promoter ribosome_entry_site feature/rbs feature/cds CDS |