BBa_J119335

BBa_J119335 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119335
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-02-03 12:00:00
Date modified: 2015-08-31 04:08:28

Ammeline Biosensor with Riboswitch M6 double prime



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_J119335_sequence (Version 1)

Description

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.

Notes

None.

Source

Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly.

Sequence Annotation Location Component / Role(s)
P5 promoter
M6'' Ammeline riboswitch
RFP
19,55
73,188
183,866
feature/promoter promoter
ribosome_entry_site feature/rbs
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119335/1