BBa_J119353

BBa_J119353 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119353
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kelly Cochran
Date created: 2014-07-06 11:00:00
Date modified: 2015-08-31 04:08:28

Ammeline Biosensor with Riboswitch N3 + RFP



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_J119353_sequence (Version 1)

Description

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).

Notes

The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).

Source

M6C"

Sequence Annotation Location Component / Role(s)
P5 promoter
N3 Ammeline riboswitch
RFP
19,55
73,190
185,868
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119353/1