BBa_J119364

BBa_J119364 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119364

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kelly Cochran, Mike Quaney

Date created: 2014-07-21 11:00:00

Date modified: 2015-08-31 04:08:28

Ammeline Fitness Module with Riboswitch N2 + TetA (T138C)

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_J119364_sequence (Version 1)

Description

The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2 with mutation T138C) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.

Notes

N2, TetA

Source

N2, TetA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 promoter	19,55	promoter, feature/promoter
Ammeline Riboswitch	73,190	ribosome_entry_site, feature/rbs
TetA	185,1382	feature/cds, CDS
T to C mutation	138,138	sequence_alteration, feature/mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119364/1

Member of these Collections

• iGEM Parts Registry