Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean Holloran
Date created: 2015-06-07 11:00:00
Date modified: 2015-08-31 04:08:29
Theophylline Riboswitch - tetracycline resistance protein (BbsI site removed)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J119385_sequence (Version 1) |
Description
Theophylline Riboswitch - tetracycline resistance proteinThis device uses a synthetic riboswitch to control tetracycline resistance. This riboswitch is modified from riboswitch D as described in the paper by Topp et al. In the presence of theophylline, the transcription of the TetA gene occurs, but in the absence of theophylline, very little transcription takes place. This part contains a modified T5 promoter, which, in the absence of cymR, acts as a strong constitutive promoter. The ribosomal binding site is contained in the riboswitch. BbsI site was removed from the TetA gene.
Reference: Shana Topp, Colleen M. K. Reynoso, Jessica C. Seeliger, Ian S. Goldlust, Shawn K. Desai, Doroth??e Murat, Aimee Shen, Aaron W. Puri, Arash Komeili, Carolyn R. Bertozzi, June R. Scott, and Justin P. Gallivan. Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species. Applied and Environmental Microbiology, 76:23, 7881-7884. December 2010.