BBa_J119395

BBa_J119395 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119395

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl

Date created: 2016-06-22 11:00:00

Date modified: 2016-06-23 03:48:27

tClone Red containing the RS3 Riboswitch

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_J119395_sequence (Version 1)

Description

The RS3 riboswitch from Wachsmuth et. al. (2013) cloned into tClone Red using Golden Gate Assembly. The RS3 riboswitch is a theophylline riboswitch that is on half of the time when theophylline is not present.

Notes

BsaI sites were used to join the RS3 riboswitch to tClone Red

Source

Wachsmuth et. al. (2013)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 Promoter	1,36	promoter, feature/promoter
RS3 Riboswitch	37,125	stem_loop, feature/stem_loop
BD18	126,213	feature/rbs, ribosome_entry_site

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_J119395/1

Member of these Collections

• iGEM Parts Registry