BBa_J119398

BBa_J119398 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119398

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony J. Eckdahl

Date created: 2016-07-11 11:00:00

Date modified: 2016-07-12 10:07:09

NiCo Riboswitch in tClone Red

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_J119398_sequence (Version 1)

Description

A riboswitch found to be responsive to transitions metals (Ni++, Co++, Mn++) from Furakawa et al. (Molecular Cell 57: 1088-1098, 2015) was cloned into tClone Red using BsaI Golden Gate Assembly.

Notes

No BsaI site in NiCo riboswitch.

Source

Synthetic oligonucleotides.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 promoter	1,36	feature/promoter, promoter
NiCo riboswitch	41,167	stem_loop, feature/stem_loop
BD18 C dog RBS	172,256	ribosome_entry_site, feature/rbs
RFP	257,937	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119398/1

Member of these Collections

• iGEM Parts Registry