BBa_J119399

BBa_J119399 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119399

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrew Hunn

Date created: 2016-07-17 11:00:00

Date modified: 2016-07-18 10:25:48

RS10 in J100270 Selection Phage

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_J119399_sequence (Version 1)

Description

Riboswith 10, from Wachsmuth et. al., inserted into J100270 selection phage between the Kanamycin resistance gene and the T7 RNAP gene. In the presence of Theophylline, this part should produce the T7 RNAP.

Notes

Inserted riboswitch via decoupled golden gate assembly.

Source

Wachsmuth et. al., J100263

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kanamycin	1,63	CDS, feature/protein
RS10 Riboswitch	64,173	feature/stem_loop, stem_loop
T7 RNAP	174,572	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119399/1

Member of these Collections

• iGEM Parts Registry