Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Norman Wang
Date created: 2011-12-03 12:00:00
Date modified: 2015-08-31 04:08:30
Protein Linker SSGSSG Library
| Types | DnaRegion |
| Roles | tag Tag |
| Sequences | BBa_J133561_sequence (Version 1) |
Description
Variant library of 6-amino-acid fusion protein linker sequences, encoded with different codon permutations while maintaining the same SSGSSG protein sequence, and the same codon usage count.Sequences from this library can be picked from when designing a construct containing multiple fusion proteins (e.g. fusing GFP to protein A, RFP to protein B, YFP to protein C, BFP to for protein D, all in the same plasmid). Select a different linker for each fusion rather than reuse multiple copies of the same linker to reduce the chance of homologous recombination/misprimining event/gibson assembly overlap similarity.
Permutations of the codons are generated while following the rules:
1. encoding only SSGSSG
2. using only codons below, where codon frequency in E. coli is represented between [], and its symbolic representation between ()
Serine: AGT(S)[8.7%], AGC(s)[16.1%], TCC($)[8.6%]
Glycine: GGC(G)[29.8%], GGT(g)[24.9%]
3. all above codons can be only used once except for (s), which is used twice
4. no two of the same codon can be next to each other, in this case no (s)(s) should occur
Library of all possible 32 permutations of protein linker sequences:
Part_Number: symbolic_representation, sequence_gapped, sequence_ungapped
BBa_J133550: s$gsSG, AGC TCC GGT AGC AGT GGC, AGCTCCGGTAGCAGTGGC
BBa_J133551: s$gSsG, AGC TCC GGT AGT AGC GGC, AGCTCCGGTAGTAGCGGC
BBa_J133552: s$GsSg, AGC TCC GGC AGC AGT GGT, AGCTCCGGCAGCAGTGGT
BBa_J133553: s$GSsg, AGC TCC GGC AGT AGC GGT, AGCTCCGGCAGTAGCGGT
BBa_J133554: sSg$sG, AGC AGT GGT TCC AGC GGC, AGCAGTGGTTCCAGCGGC
BBa_J133555: sSgs$G, AGC AGT GGT AGC TCC GGC, AGCAGTGGTAGCTCCGGC
BBa_J133556: sSG$sg, AGC AGT GGC TCC AGC GGT, AGCAGTGGCTCCAGCGGT
BBa_J133557: sSGs$g, AGC AGT GGC AGC TCC GGT, AGCAGTGGCAGCTCCGGT
BBa_J133558: $sGsSg, TCC AGC GGC AGC AGT GGT, TCCAGCGGCAGCAGTGGT
BBa_J133559: $sGSsg, TCC AGC GGC AGT AGC GGT, TCCAGCGGCAGTAGCGGT
BBa_J133560: $sgsSG, TCC AGC GGT AGC AGT GGC, TCCAGCGGTAGCAGTGGC
BBa_J133561: $sgSsG, TCC AGC GGT AGT AGC GGC, TCCAGCGGTAGTAGCGGC
BBa_J133562: $sGSsg, TCC AGC GGC AGT AGC GGT, TCCAGCGGCAGTAGCGGT
BBa_J133563: $sGsSg, TCC AGC GGC AGC AGT GGT, TCCAGCGGCAGCAGTGGT
BBa_J133564: $sgSsG, TCC AGC GGT AGT AGC GGC, TCCAGCGGTAGTAGCGGC
BBa_J133565: $sgsSG, TCC AGC GGT AGC AGT GGC, TCCAGCGGTAGCAGTGGC
BBa_J133566: s$gsSG, AGC TCC GGT AGC AGT GGC, AGCTCCGGTAGCAGTGGC
BBa_J133567: s$gSsG, AGC TCC GGT AGT AGC GGC, AGCTCCGGTAGTAGCGGC
BBa_J133568: s$GsSg, AGC TCC GGC AGC AGT GGT, AGCTCCGGCAGCAGTGGT
BBa_J133569: s$GSsg, AGC TCC GGC AGT AGC GGT, AGCTCCGGCAGTAGCGGT
BBa_J133570: sSg$sG, AGC AGT GGT TCC AGC GGC, AGCAGTGGTTCCAGCGGC
BBa_J133571: sSgs$G, AGC AGT GGT AGC TCC GGC, AGCAGTGGTAGCTCCGGC
BBa_J133572: sSG$sg, AGC AGT GGC TCC AGC GGT, AGCAGTGGCTCCAGCGGT
BBa_J133573: sSGs$g, AGC AGT GGC AGC TCC GGT, AGCAGTGGCAGCTCCGGT
BBa_J133574: Ssgs$G, AGT AGC GGT AGC TCC GGC, AGTAGCGGTAGCTCCGGC
BBa_J133575: Ssg$sG, AGT AGC GGT TCC AGC GGC, AGTAGCGGTTCCAGCGGC
BBa_J133576: SsGs$g, AGT AGC GGC AGC TCC GGT, AGTAGCGGCAGCTCCGGT
BBa_J133577: SsG$sg, AGT AGC GGC TCC AGC GGT, AGTAGCGGCTCCAGCGGT
BBa_J133578: Ssg$sG, AGT AGC GGT TCC AGC GGC, AGTAGCGGTTCCAGCGGC
BBa_J133579: Ssgs$G, AGT AGC GGT AGC TCC GGC, AGTAGCGGTAGCTCCGGC
BBa_J133580: SsG$sg, AGT AGC GGC TCC AGC GGT, AGTAGCGGCTCCAGCGGT
BBa_J133581: SsGs$g, AGT AGC GGC AGC TCC GGT, AGTAGCGGCAGCTCCGGT