BBa_J15001

BBa_J15001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J15001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-07-12 11:00:00
Date modified: 2015-08-31 04:08:32

strong synthetic E. coli ribosome binding site with SacI site.



Types
DnaRegion

Roles
RBS

ribosome_entry_site

Sequences BBa_J15001_sequence (Version 1)

Description

This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.

Notes

Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.

Source

Synthetic.

Sequence Annotation Location Component / Role(s)
SacI
rbs
1,3
4,10
sequence_feature feature/misc
feature/rbs ribosome_entry_site
igem#experience None  
igem#sampleStatus Not in stock  
igem#status Unavailable  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_J15001/1