Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jara Radeck
Date created: 2013-12-20 12:00:00
Date modified: 2015-08-31 04:08:35
pBs4S: Bacillus subtilis vector, thrC integration, spec resistance
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_J179002_sequence (Version 1) |
Description
This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. This backbone is a BioBricked version of the B. subtilis vector pDG1731.Notes
This vector is compatible with pBS1C and pBS2E in one strain.Source
This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBoxFor handling of B. subtilis vectors, please see here.
The transformation into B. subtilis is explained here.
| Sequence Annotation | Location | Component / Role(s) |
| spc thrC,thrB back ori bla hom, trC front | 211,885 1028,1863 1942,2556 2716,3576 3731,4542 | CDS feature/cds feature/misc sequence_feature sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc |