HisTag

BBa_J18909 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18909

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-15 12:00:00

Date modified: 2015-08-31 04:08:35

His tag (6xHis
E. coli - optimized)

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_J18909_sequence (Version 1)

Description

Hexahistidine affinity tag.

Binds zinc, nickel, or cobalt affinity resins.

=Notes=

This part is identical to BBa_I757013 (which does not seem to be available though).

=References=

Wikipedia article: http://en.wikipedia.org/wiki/Polyhistidine-tag

Notes

Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.

Source

Synthetic DNA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PolyHis	1,18	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18909/1

Member of these Collections

• //proteindomain/affinity
• iGEM Parts Registry