BBa_J18911

BBa_J18911 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J18911
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-25 12:00:00
Date modified: 2015-08-31 04:08:36

RFC 25 vector reverse (prefix)



Types
DnaRegion

Roles
Primer

primer

Sequences BBa_J18911_sequence (Version 1)

Description

Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 suffix but pointing outward into the backbone rather than insert.

Annealing temperature: 68 C (in Phusion polymerase buffer)

Example vectors:
pSB1AC3F, pSB1AK3F, pSB1AT3F, pMA

Notes

optimized to be used with BBa_J18910

Source

designed

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J18911/1