BBa_J18911

BBa_J18911 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J18911
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-25 12:00:00
Date modified: 2015-08-31 04:08:36

RFC 25 vector reverse (prefix)



    • Details

    Types
    DnaRegion

    Roles
    Primer

    primer

    Sequences BBa_J18911_sequence (Version 1)

    Description

    Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 suffix but pointing outward into the backbone rather than insert.

    Annealing temperature: 68 C (in Phusion polymerase buffer)

    Example vectors:
    pSB1AC3F, pSB1AK3F, pSB1AT3F, pMA

    Notes

    optimized to be used with BBa_J18910

    Source

    designed

    igem#experience
    Works
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J18911/1
     

    Attachments

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