StrepII

BBa_J18916 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J18916
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-26 12:00:00
Date modified: 2015-08-31 04:08:36

Strep tag II



    • Details

    Types
    DnaRegion

    Roles
    Tag

    tag

    Sequences BBa_J18916_sequence (Version 1)

    Description

    Protein purification tag. Bound by Strepavidine (optimized: strepTactin(tm)) and eluted with biotin. Allows for mild and efficient elution.

    SEE ALSO:

    • novagen.com, gehealthcare.com (former amersham) for purification protocols


    • BBa_I757014, StrepTag II from Freiburg iGem team, has three codon variations


    Notes

    • gene synthesis


    • codon optimized for E. coli


    Source

    pET52b

    gene synthesis

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J18916/1
     

    Attachments

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