| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_J202005_sequence (Version 1)
|
Description
This is PBAD (BBa_I13453) mutant. -67 relative to the araC transcriptional start site was changed from T to A.
Notes
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.
Source
This mutant was created by site-directed mutagenesis.
