BBa_J202047

BBa_J202047 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J202047

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jiyeon Park

Date created: 2012-07-26 11:00:00

Date modified: 2015-08-31 04:08:37

Ptet Characterization (Wild-type)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_J202047_sequence (Version 1)

Description

This part can be used as a wild-type control to characterize other Ptet mutants.

Notes

TetR(C0040) is expressed under constitutive promoter (J23101). TetR binds O2 sites in Ptet, and binding can be measured by GFP expression.

Source

Built from existing parts in the registry though BioBrick assembly.

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_E0240	GFP report
public	BBa_J23101	BBa_J23101
public	BBa_B0032	BBa_B0032
public	BBa_C0040	tetR
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_E0240	63,938	GFP report
BBa_J23101	947,981	BBa_J23101
BBa_B0032	990,1002	BBa_B0032
BBa_C0040	1009,1693	tetR
BBa_B0015	1702,1830	BBa_B0015

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J202047/1

Member of these Collections

• iGEM Parts Registry