BBa_J22102

BBa_J22102 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J22102

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Krithiga Sankaran

Date created: 2006-09-03 11:00:00

Date modified: 2015-08-31 04:08:38

rec A (SOS) Promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory

Description

The rec A (SOS)promoter

Notes

this is the description of the primers used for PCR amplification
Primer Number 2a, Forward Primer (E-N-X)

GAATTCGCGGCCGCTTCTAG AACAATTTCTACAAAACACTTGATACTGT

Tm: 59.43
Length: 29
GC%:27.59


Primer 2b, Reverse Primer (P-N-S)

CTGCAGGCGGCCGAACTAGTT GCATGATGGAGCCTTTACC

Tm: 55.2
Length: 19
GC%: 56.63

Source

The Rec A(SOS) promoter from the chromosomal DNA from MG1655(E coli) has been PCR amplified and inserted into plasmid pSB1A2 (part number BBa_B0015).

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J22102/1

Member of these Collections

• iGEM Parts Registry