BBa_J23005

BBa_J23005 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_J23005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-06-22 11:00:00
Date modified: 2015-08-31 04:08:38

[FRT-CmR-FRT]



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_J23005_sequence (Version 1)

    Description

    Biobricked cassette containing the chloramphenicol resistance gene flanked by FRT sites. Useful for constructing markerless genome-integration cassettes. The cassette is derived from pKD3 described by Datsenko&Wanner and can be excised by expression of flp recombinase in trans with helper plasmids such as pCP20.

    Notes

    N/A

    Source


    PCR ca1005F/R on pKD3 (1066 bp, EcoRI/PstI)

    sub into pSB1A2 (EcoRI/PstI)

    product is pSB1A2-Bca1005


    ca1005F Forward Biobrick for pKD3 FRT-Cm-FRT

    gacttgaattcgcggccgcttctagagtgtaggctggagctgcttc

    ca1005R Reverse Biobrick for pKD3 FRT-Cm-FRT

    cctaactgcagcggccgctactagtacatatgaatatcctccttag




    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J23005/1
     

    Attachments

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