BBa_J23012

BBa_J23012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Will Bosworth
Date created: 2006-07-23 11:00:00
Date modified: 2015-08-31 04:08:38

Spectinomycin R open-reading-frame basic part



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_J23012_sequence (Version 1)

Description

it is a Spec resistant biobrick

Notes

JL1 Forward EcoRI oligo to bioBrick SpecR:
gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc
JL2 Reverse SpeI oligo to bioBrick SpecR:
ccgctactagtCAGCAACGATGTTACGCAGC

Source

PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)

Sequence Annotation Location Component / Role(s)
SpR Spectinomycin Resistance
46,837
CDS feature/cds
igem#experience
Issues
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J23012/1