Types | DnaRegion
|
Roles | engineered_region
Generator
|
Sequences | BBa_J23012_sequence (Version 1)
|
Description
it is a Spec resistant biobrick
Notes
JL1 Forward EcoRI oligo to bioBrick SpecR:
gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc
JL2 Reverse SpeI oligo to bioBrick SpecR:
ccgctactagtCAGCAACGATGTTACGCAGC
Source
PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)