BBa_J23015

BBa_J23015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Liang
Date created: 2006-07-23 11:00:00
Date modified: 2015-08-31 04:08:39

F plasmid pOX38/MC1061 oriTf knockout,(pOX38
oriTf::CmR)



    • Details

    Types
    DnaRegion

    Roles
    Cell

    chromosome

    Description

    F plasmid with OriT knockout

    Notes

    ForlamF Forward oligo to knockout OriT:
    tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc
    ForlamR Reverse oligo to knockout OriT:
    ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa

    Source

    PCR FORlamF/ForlamR on pKD3 (~1000bp, dPN1, Neb2)
    Electroporate into pKD46xpOX38

    igem#experience
    Works
     
    igem#sampleStatus
    No part sequence
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J23015/1
     

    Attachments

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