BBa_J23015

BBa_J23015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Liang
Date created: 2006-07-23 11:00:00
Date modified: 2015-08-31 04:08:39

F plasmid pOX38/MC1061 oriTf knockout,(pOX38
oriTf::CmR)



Types
DnaRegion

Roles
Cell

chromosome

Description

F plasmid with OriT knockout

Notes

ForlamF Forward oligo to knockout OriT:
tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc
ForlamR Reverse oligo to knockout OriT:
ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa

Source

PCR FORlamF/ForlamR on pKD3 (~1000bp, dPN1, Neb2)
Electroporate into pKD46xpOX38

igem#experience
Works
 
igem#sampleStatus
No part sequence
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J23015/1