BBa_J23068

BBa_J23068 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J23068

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2006-08-13 11:00:00

Date modified: 2015-08-31 04:08:39

Intermediate #2 in construction of TraGr cassette

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_J23068_sequence (Version 1)

Description

TraG region (J23068) cloned into pSB1A2-Bca9007

Notes

n/a

Source

PCR JL20/JL21 on RP4 gen. (2268 bp, MfeI/BglII)

Sub into pGLN (BamHI/EcoRI)

Product is pJL20(J23067)

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IPCR JL22/JL23 on pJL20(J23067) (3454 bp, EcoRI/PstI)

Sub in pSB1A2-Bca9007 (1015 bp, EcoRI/PstI)

Product is pJL22(J23068)

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PCR JL18/JL19 on pJL22(J23068) for D/W (bp, DpnI)

Datsenko Wanner on pKD46/Rlambda,

Product is J23069

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JL20 Forward BglII to BamHI for traG-5' knockout cassette

GAGTCAGATCTgattatgttggtgcgctg

JL21 Reverse MfeI to EcoRI for traG-5' knockout cassette

gctggCAATTGcattcgatttccagtacaggc

JL22 Reverse EcoRI IPCR of traG

gctgcGAATTCgatactggaaggaatgggc

JL23 Forward PstI IPCR of traG

cattgCTGCAGacggcagcattgaagaggcg

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J23068/1

Member of these Collections

• iGEM Parts Registry