Types | DnaRegion
|
Roles | Plasmid
plasmid
|
Sequences | BBa_J23076_sequence (Version 1)
|
Description
This is a p15A/CmR plasmid designed for shortcuts to promoter reporters with RFP, or as a shortcut to pSB3C6 variants with the r0040 Ptet promoter part. The EcoRI/PstI region goes:
E-X-[Ptet]-S-[stRBS][RFP][TT]-P
Notes
N/A
Source
JCA082006-Construction of pSB3C6-Bca1020/r0040=J23076
Digest pBca1020-r0040 . . . . . (EcoRI/PstI, 966 bp, small)
Sub into pAC899 . . . . . . . . (EcoRI/PstI)
Product is pJ23076, temp is pSB3C6-Bca1020