mRFPr

BBa_J31008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J31008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Karmella Haynes
Date created: 2007-02-15 12:00:00
Date modified: 2015-08-31 04:08:45

RFP reverse



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_J31008_sequence (Version 1)

    Description

    The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed

    Notes

    RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers:
    5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F"
    5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"

    Source

    RFP reverse was PCR amplified from mRFP (BBaE1010).

    Sequence Annotation Location Component / Role(s)
    mRFP reverse
    Spe mRFP F
    Xba mRFP R
    		1,678
    659,678
    1,20
    		CDS feature/cds
    primer_binding_site feature/primer_binding
    primer_binding_site feature/primer_binding

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J31008/1
     

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