BBa_J32009

BBa_J32009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J32009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austen Heinz
Date created: 2006-07-15 11:00:00
Date modified: 2015-08-31 04:08:45

4HT Dependent Intein



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_J32009_sequence (Version 1)

    Description

    Intein splices itself out of a protein placed on N and C terminal ends in the presence of 4HT allowing for control protein activity in a dose dependent manner.

    Notes

    Removal of multiple BB restriction sites.

    Source

    Allen R. Buskirk, Yi-Ching Ong, Zev J. Gartner, and David R. Liu "Directed evolution of ligand dependence: Small-molecule-activated protein splicing" PNAS 101: 10505-10510

    Sequence Annotation Location Component / Role(s)
    J32009
    4-HT dependent intein
    		1,1233
    1,1233
    		feature/BioBrick engineered_region
    CDS feature/cds

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J32009/1
     

    Attachments

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