BBa_J36836

BBa_J36836 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J36836
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perry Tsai
Date created: 2006-10-28 11:00:00
Date modified: 2015-08-31 04:08:47

Outer membrane protein A, aa 46-66



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_J36836_sequence (Version 1)

Description

This part codes for amino acids #46-66 of outer membrane protein A (OmpA). It corresponds to a single transmembrane domain of OmpA, crossing the outer membrane of E. coli.

This was fused with the lipoprotein signal peptide upstream and streptavidin downstream in the project to express streptavidin on the outer surface of E. coli.

Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Source

E. coli K12

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J36836/1