BBa_J36837

BBa_J36837 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J36837

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Perry Tsai

Date created: 2006-10-28 11:00:00

Date modified: 2015-08-31 04:08:47

Outer membrane protein A, aa 46-159

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J36837_sequence (Version 1)

Description

This part codes for amino acids #46-159 of outer membrane protein A (OmpA). It corresponds to five transmembrane domains of OmpA, crossing the outer membrane of E. coli.

This was fused with the lipoprotein signal peptide upstream and streptavidin downstream in the project to express streptavidin on the outer surface of E. coli.

Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Source

E. coli K12

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J36837/1

Member of these Collections

• iGEM Parts Registry