BBa_J40003

BBa_J40003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J40003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Horia Vulpe
Date created: 2006-10-24 11:00:00
Date modified: 2015-08-31 04:08:48

LacI (LVA) + ECFP (LVA) under luxpR promoter + Brickalator 2.0



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_J40003_sequence (Version 1)

    Description

    Polycistronic LacI and ECFP proteins with degradation tags regulated by luxpR promoter (expression up-regulated by LuxR/HSL complex) ligated to I5612 Brickalator 2.0 (Output- Repressilator). Used as a part in a quorum sensing stabilized repressilator system. Allows to select for both bricks with ampicillin.

    Notes

    Standard ligation

    Source

    source: iGEM 2006

    Access Instance Definition
    public
    public
    		BBa_J40001
    BBa_I5612
    		BBa_J40001
    BBa_I5612

    Sequence Annotation Location Component / Role(s)
    BBa_J40001
    BBa_I5612
    		1,2159
    2168,5443
    		BBa_J40001
    BBa_I5612

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J40003/1
     

    Attachments

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