hixC

BBa_J44000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J44000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Missouri Western and Davidson Groups, Todd Eckdahl

Date created: 2006-06-05 11:00:00

Date modified: 2015-08-31 04:08:48

hixC binding site for Salmonella typhimurium Hin recombinase

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J44000_sequence (Version 1)

Description

A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.

Notes

Standard BioBrick prefix and suffix were added to the 26 bp sequence.

Source

Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase
http://www.genetics.org/cgi/content/abstract/149/4/1649

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J44000/1

Member of these Collections

• //function/recombination/hin
• iGEM Parts Registry