Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MIT IGEM 2006
Date created: 2006-08-20 11:00:00
Date modified: 2015-08-31 04:08:49
isochorismate pyruvate-lyase and isochorismate synthase (pchBA)
converts chorismate to salicylate
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J45017_sequence (Version 1) |
Description
pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.Notes
n/aSource
pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.| Sequence Annotation | Location | Component / Role(s) |
| g -> a to eliminate PstI site pchA start codon pchB start codon pchB stop codon pchA double TAA stop codon PchB PchA | 440,440 303,305 1,3 304,306 1731,1736 1,306 303,1736 | point_mutation feature/s_mutation feature/start start_codon feature/start start_codon stop_codon feature/stop feature/stop stop_codon CDS feature/cds CDS feature/cds |