p(osmY)

BBa_J45993 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J45993

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stephen Payne

Date created: 2006-07-10 11:00:00

Date modified: 2015-08-31 04:08:50

Minimal stationary phase osmY promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_J45993_sequence (Version 1)

Description

osmY's promoter is active in stationary phase and under high osmotic pressure conditions.

Notes

Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'

Total Length: 49 bp

Annealing Length: 21 bp

GC Content: 38.1%

Melting Temperature: 49.5 degrees C

hairpin deltaG: -2.66 kcal/mol

self dimer deltaG: -99.97 kcal/mol

Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'

Total Length: 54 bp

Annealing Length: 25 bp

GC Content: 20.0%

Melting Temperature: 46.4 degrees C

hairpin deltaG: -2.86 kcal/mol

self dimer deltaG: -99.44 kcal/mol

heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol

Source

This part was PCRed out of E. coli's genome.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	46,51	promoter, feature/promoter
-35	22,27	promoter, feature/promoter

Other Properties

• igem#experience: Fails
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J45993/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/constitutive
• //rnap/prokaryote/ecoli/sigmas
• //s
• iGEM Parts Registry